About MS ASSETs Glycan

“MS ASSETs Glycan” is a simple web application that estimates possible glycan compositions from the peak m/z value obtained by an MS 1 measurement of the glycan. This application is developed and operated by Shimadzu Corporation, and is available for free.

This application does not require a glycan database to estimate the glycan composition. A brute-force search is carried out on all glycans obtained by combining the monosaccharides selected by users to find candidate glycan compositions that match the peaks.

In addition, an optional functionality is provided to handle changes in the mass of sialic acid residues produced by various sialic acid derivatization methods. This functionality supports newly developed methods such as SALSA (1 - 3) and other sialic acid linkage-specific derivatization methods for distinguishing linkage isomers of sialic acids on glycans using mass spectrometry.

When a sialic acid linkage-specific derivatization is performed, not only the number of detected peaks but also the number of candidate glycan compositions for each peak are likely to increase. Candidates can be narrowed down by using the unique Sialic filter function.

The optinal function for sialic acid derivatization is paid option for a customer of SialoCapper TM -ID Kit.

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1(1,2)

Nishikaze, T.; Tsumoto, H.; Sekiya, S.; Iwamoto, S.; Miura, Y.; Tanaka, K. Differentiation of Sialyl Linkage Isomers by One-Pot Sialic Acid Derivatization for Mass Spectrometry-Based Glycan Profiling. Anal. Chem. 2017, 89, 2353–2360. https://doi.org/10.1021/acs.analchem.6b04150

2

Hanamatsu, H.; Nishikaze, T.; Miura, N.; Piao, J.; Okada, K.; Sekiya, S.; Iwamoto, S.; Sakamoto, N.; Tanaka, K.; Furukawa, J. Sialic Acid Linkage Specific Derivatization of Glycosphingolipid Glycans by Ring-Opening Aminolysis of Lactones. Anal. Chem. 2018, 90 (22), 13193–13199. https://doi.org/10.1021/acs.analchem.8b02775.

3(1,2)

Nishikaze, T. Sialic Acid Derivatization for Glycan Analysis by Mass Spectrometry. Proc. Japan Acad. Ser. B 2019, 95 (9), 523–537. https://doi.org/10.2183/pjab.95.036.